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I think so, becuase when Santana, Brittany, Mercedes, Kurt, etc. were on a conference call in season one, talking about how they had to find some way to keep Rachel from telling Finn the truth, they said something about how sex doesn't mean your dating, and Brittany said "Good, cause that'll mean Santana and I were dating". Hope I helped!
Also, they've seen having sex during Duets